Isolation of Actinomycetes from Marine Sponges and Hawaiian Sediments
Gerre Dias , Towson University; Julie Enticknap and Dr. Russell Hill, Center of Marine Biotechnology University of Maryland
Actinomycete bacteria are very versatile. They produce many bioactive compounds, including 60% of naturally occurring antibiotics, but there are additional unknown bacteria that have yet to be discovered. Marine sponges and Hawaiian sediments were collected for molecular analysis of the prokaryotic community contained within them looking specifically for novel actinomycete bacteria. Identification of actinomycetes involves DNA analysis. Several samples of Microciona prolifera were brought to the lab, some were freeze dried for DNA analysis and others were kept alive for life sustaining methods. DNA was extracted from Microciona prolifera using a bead beater method. This method has been shown to work on several sponges in our lab. DNA from these sponges were amplified using 16S rDNA primers. A denaturing gel gradient electrophoresis(DGGE) and PCR amplification was done on the samples. Similar methods were performed for extraction and amplification of DNA from Hawaiian sediments

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